It is a two-bay suburban summerhouse with a porch and a log construction, covered by a shingled, hipped roof. The building, erected by Leonard Serafiński in the 1850s near Wiśnicz Castle and in the hands of the Serafiński family since its construction, has retained its original appearance and furnishings.

Jan Matejko often came here to visit Joanna and Leonard Serafiński, meeting friends from his school years and his fiancée Teodora, Joanna's younger sister. The Serafiński family took care of the painter's memorabilia for several generations, with occasional access between the wars. During the Second World War, as part of the underground resistance, Ludmiła and Tomasz Serafiński carried out secret activities in the manor. They hid people wanted by the Nazis, including Witold Pilecki who had escaped from Auschwitz. In 2008, his memorial plaque was unveiled in front of the museum. 

After the war, Tomasz Serafiński made the memorabilia and interiors of the manor house seasonally available to visitors. In 1976, renovation of the building began, and in 1981, through the owners' efforts, the museum was opened as a branch of the Museum of the Tarnów Region. The museum is housed at the front in the lounge and study of Stanisława Serafińska, the author of a memoir dedicated to Matejko. The exhibition includes furniture and furnishings from the 18th to 19th centuries, paintings, sketches and drawings (including portraits of the Giebułtowskis and caricatures of the manor's inhabitants), and Matejko's piano and other related objects. A garden with beehives surrounds the manor house.